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This technique can be used in conjunction with laser diffraction to gain a deeper understanding of the sample or to validate the laser diffraction method. One of the main advantages of automated imaging is that it provides statistically representative distributions by measuring 10’s to 100’s of thousands of particles per measurement. As a result, particle size distribution and particle morphology are determined. Individual particle images are captured from dispersed sample and scanned across sample area underneath the microscope optics while keeping the particles in focus.
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It can characterize particles ranging from 0.5 microns to several millimeters in size. Malvern Morphologi G3SE - high resolution microscope-based automated imaging system Mastersizer 3000 with dry and wet sample dispersion units This technique provides rapid measurements (several minutes) and repeatability. In laser diffraction a volume distribution is obtained, which shows the volume percentage of particles at a given size obtained. The angular scattering intensity received is then analyzed to calculate the size of the particles responsible for creating the scattering pattern. Laser diffraction works by measuring the angular variation in intensity of light scattered as a laser beam passes through a well dispersed sample. In the case of laser diffraction systems, the particle size reported is the diameter of a sphere that yields an equivalent light scattering pattern as the particle being measured. Malvern Mastersizer 3000 - Laser Diffraction (LD)
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